In vitro release of vascular endothelial growth factor from gadolinium-doped biodegradable microspheres.

Published

Journal Article

A drug delivery vehicle was constructed that could be visualized noninvasively with MRI. The biodegradable polymer poly(DL-lactic-co-glycolic acid) (PLGA) was used to fabricate microspheres containing vascular endothelial growth factor (VEGF) and the MRI contrast agent gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA). The microspheres were characterized in terms of size, drug and contrast agent encapsulation, and degradation rate. The PLGA microspheres had a mean diameter of 48 +/- 18 microm. The gadolinium loading was 17 +/- 3 microg/mg polymer and the VEGF loading was 163 +/- 22 ng/mg polymer. Electron microscopy revealed that the Gd was dispersed throughout the microspheres and it was confirmed that the Gd loading was sufficient to visualize the microspheres under MRI. VEGF and Gd-DTPA were released from the microspheres in vitro over a period of approximately 6 weeks in three phases: a burst, followed by a slow steady-state, then a rapid steady-state. Biodegradable Gd-doped microspheres can be effectively used to deliver drugs in a sustained manner, while being monitored noninvasively with MRI.

Full Text

Cited Authors

  • Faranesh, AZ; Nastley, MT; Perez de la Cruz, C; Haller, MF; Laquerriere, P; Leong, KW; McVeigh, ER

Published Date

  • June 2004

Published In

Volume / Issue

  • 51 / 6

Start / End Page

  • 1265 - 1271

PubMed ID

  • 15170848

Pubmed Central ID

  • 15170848

Electronic International Standard Serial Number (EISSN)

  • 1522-2594

International Standard Serial Number (ISSN)

  • 0740-3194

Digital Object Identifier (DOI)

  • 10.1002/mrm.20092

Language

  • eng