Ocular nanoparticle toxicity and transfection of the retina and retinal pigment epithelium.


Journal Article

Chitosan, PCEP (poly{[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium iodide] ethyl phosphate}), and magnetic nanoparticles (MNPs) were evaluated for the safe delivery of genes in the eye. Rabbits were injected with nanoparticles either intravitreally (IV) or subretinally (SR) and sacrificed 7 days later. Eyes were grossly evaluated for retinal pigment epithelium abnormalities, retinal degeneration, and inflammation. All eyes were cryopreserved and sectioned for analysis of toxicity and expression of either enhanced green or red fluorescent proteins. All of the nanoparticles were able to transfect cells in vitro and in vivo. IV chitosan showed inflammation in 12/13 eyes, whereas IV PCEP and IV MNPs were not inflammatory and did not induce retinal pathology. SR PCEP was nontoxic in the majority of cases but yielded poor transfection, whereas SR MNPs were nontoxic and yielded good transfection. Therefore, we conclude that the best nanoparticle evaluated in vivo was the least toxic nanoparticle tested, the MNP.

Full Text

Cited Authors

  • Prow, TW; Bhutto, I; Kim, SY; Grebe, R; Merges, C; McLeod, DS; Uno, K; Mennon, M; Rodriguez, L; Leong, K; Lutty, GA

Published Date

  • December 2008

Published In

Volume / Issue

  • 4 / 4

Start / End Page

  • 340 - 349

PubMed ID

  • 18640079

Pubmed Central ID

  • 18640079

Electronic International Standard Serial Number (EISSN)

  • 1549-9642

International Standard Serial Number (ISSN)

  • 1549-9634

Digital Object Identifier (DOI)

  • 10.1016/j.nano.2008.06.003


  • eng