In vitro chondrogenesis of mesenchymal stem cells in recombinant silk-elastinlike hydrogels.


Journal Article

In this study the chondrocytic differentiation and cartilage matrix accumulation of human mesenchymal stem cells (hMSCs) were investigated after encapsulation in a genetically engineered silk-elastinlike protein polymer SELP-47 K as an injectable matrix for delivery of cell-based therapeutics.hMSCs were encapsulated in SELP-47 K and cultured for 4 weeks in chondrogenic medium with or without transforming growth factor-beta3 (TGF). Chondrogenic differentiation was evaluated by histological, RNA and biochemical analyses for the expression of cartilage extracellular matrix components.Histological and immunohistochemical staining revealed that the cells acquired a rounded morphology and were embedded in significant amounts of chondrogenic extracellular matrix. Reverse transcriptase (RT)-PCR showed an up-regulation in aggrecan, type II and type X collagen and SOX9 in presence of TGF-beta3. By day 28, constructs cultured in the presence of TGF-beta3 exhibited significant increase in sulfated glycosaminoglycan and total collagen content up to 65 and 300%, respectively.This study demonstrates that SELP-47 K hydrogel can be used as a scaffold for encapsulation and chondrogenesis of hMSCs. The ability to use recombinant techniques to precisely control SELP structure enables the investigation of injectable protein polymer scaffolds for soft-tissue engineering with varied physicochemical properties.

Full Text

Cited Authors

  • Haider, M; Cappello, J; Ghandehari, H; Leong, KW

Published Date

  • March 2008

Published In

Volume / Issue

  • 25 / 3

Start / End Page

  • 692 - 699

PubMed ID

  • 17404809

Pubmed Central ID

  • 17404809

Electronic International Standard Serial Number (EISSN)

  • 1573-904X

International Standard Serial Number (ISSN)

  • 0724-8741

Digital Object Identifier (DOI)

  • 10.1007/s11095-007-9282-8


  • eng