Myogenic induction of aligned mesenchymal stem cell sheets by culture on thermally responsive electrospun nanofibers


Journal Article

A study was conducted to demonstrate the ability of electrospun aligned nanofibers composed of thermally responsive hydroxybutyl chitosan, to create a robust scaffold for producing aligned cell sheets. It was found that aligned nanofibers created spaced nanometer-scale topographical features on the scaffold surface. The topographical features induced gene expression, which indicated myogenic induction of human mesenchymal stem cells (hMSC) cultured in proliferative and nondifferentiatng medium. Thermal reversibility of the fibers allowed dissolution of the polymer from the cell or scaffold construct, without disrupting cytosskeletal structure and cell-cell interactions. It was observed that the thermally reversible nanofibers can produce polymer-free cell layers, engineered with exposure to nanotopographical cues.

Full Text

Cited Authors

  • Dang, JM; Leong, KW

Published Date

  • October 5, 2007

Published In

Volume / Issue

  • 19 / 19

Start / End Page

  • 2775 - 2779

International Standard Serial Number (ISSN)

  • 0935-9648

Digital Object Identifier (DOI)

  • 10.1002/adma.200602159

Citation Source

  • Scopus