Bombesin-like peptides and mast cell responses: relevance to bronchopulmonary dysplasia?

Published

Journal Article

Bombesin-like peptides (BLPs) are elevated in newborns who later develop bronchopulmonary dysplasia (BPD). In baboon models, anti-BLP blocking antibodies abrogate BPD. We now demonstrate hyperplasia of both neuroendocrine cells and mast cells in lungs of baboons with BPD, compared with non-BPD controls or BLP antibody-treated BPD baboons. To determine whether BLPs are proinflammatory, bombesin was administered intratracheally to mice. Forty-eight hours later, we observed increased numbers of lung mast cells. We analyzed murine mast cells for BLP receptor gene expression, and identified mRNAs encoding bombesin receptor subtype 3 and neuromedin-B receptor (NMB-R), but not gastrin-releasing peptide receptor. Only NMB-R-null mice accumulated fewer lung mast cells after bombesin treatment. Bombesin, gastrin-releasing peptide, NMB, and a bombesin receptor subtype 3-specific ligand induced mast cell proliferation and chemotaxis in vitro. These observations support a role for multiple BLPs in promoting mast cell responses, suggesting a mechanistic link between BLPs and chronic inflammatory lung diseases.

Full Text

Duke Authors

Cited Authors

  • Subramaniam, M; Sugiyama, K; Coy, DH; Kong, Y; Miller, YE; Weller, PF; Wada, K; Wada, E; Sunday, ME

Published Date

  • September 1, 2003

Published In

Volume / Issue

  • 168 / 5

Start / End Page

  • 601 - 611

PubMed ID

  • 12807697

Pubmed Central ID

  • 12807697

International Standard Serial Number (ISSN)

  • 1073-449X

Digital Object Identifier (DOI)

  • 10.1164/rccm.200212-1434OC

Language

  • eng

Conference Location

  • United States