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Calcitonin driven v-Ha-ras induces multilineage pulmonary epithelial hyperplasias and neoplasms.

Publication ,  Journal Article
Sunday, ME; Haley, KJ; Sikorski, K; Graham, SA; Emanuel, RL; Zhang, F; Mu, Q; Shahsafaei, A; Hatzis, D
Published in: Oncogene
July 29, 1999

We initiated a transgenic model for primary pulmonary neuroendocrine cell (PNEC) hyperplasia/neoplasia using v-Ha-ras driven by the neural/neuroendocrine (NE)-specific calcitonin promoter (rascal). Previously, we showed that nitrosamine treated rodents develop PNEC hyperplasia but non-NE lung tumors, with variable outcomes presumably reflecting ras activation in multiple cell lineages. Interestingly, all rascal transgenic mouse lineages develop hyperplasias of NE and non-NE cells but mostly non-NE lung carcinomas, with rascal mRNA in differentiated PNECs and tumor cells. Analyses of embryonic lung demonstrate rascal mRNA in undifferentiated epithelium, consistent with expression in a common pluripotent precursor cell. These unexpected observations indicate that v-Ha-ras can lead to both NE and non-NE hyperplasia/neoplasia in vivo, opening new avenues for studies of lung carcinogenesis.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

July 29, 1999

Volume

18

Issue

30

Start / End Page

4336 / 4347

Location

England

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Neuroendocrine Tumors
  • Mice, Inbred C57BL
  • Mice
  • Lung Neoplasms
  • Lung
  • In Situ Hybridization
  • Immunohistochemistry
 

Citation

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Sunday, M. E., Haley, K. J., Sikorski, K., Graham, S. A., Emanuel, R. L., Zhang, F., … Hatzis, D. (1999). Calcitonin driven v-Ha-ras induces multilineage pulmonary epithelial hyperplasias and neoplasms. Oncogene, 18(30), 4336–4347. https://doi.org/10.1038/sj.onc.1202810
Sunday, M. E., K. J. Haley, K. Sikorski, S. A. Graham, R. L. Emanuel, F. Zhang, Q. Mu, A. Shahsafaei, and D. Hatzis. “Calcitonin driven v-Ha-ras induces multilineage pulmonary epithelial hyperplasias and neoplasms.Oncogene 18, no. 30 (July 29, 1999): 4336–47. https://doi.org/10.1038/sj.onc.1202810.
Sunday ME, Haley KJ, Sikorski K, Graham SA, Emanuel RL, Zhang F, et al. Calcitonin driven v-Ha-ras induces multilineage pulmonary epithelial hyperplasias and neoplasms. Oncogene. 1999 Jul 29;18(30):4336–47.
Sunday, M. E., et al. “Calcitonin driven v-Ha-ras induces multilineage pulmonary epithelial hyperplasias and neoplasms.Oncogene, vol. 18, no. 30, July 1999, pp. 4336–47. Pubmed, doi:10.1038/sj.onc.1202810.
Sunday ME, Haley KJ, Sikorski K, Graham SA, Emanuel RL, Zhang F, Mu Q, Shahsafaei A, Hatzis D. Calcitonin driven v-Ha-ras induces multilineage pulmonary epithelial hyperplasias and neoplasms. Oncogene. 1999 Jul 29;18(30):4336–4347.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

July 29, 1999

Volume

18

Issue

30

Start / End Page

4336 / 4347

Location

England

Related Subject Headings

  • Reverse Transcriptase Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Neuroendocrine Tumors
  • Mice, Inbred C57BL
  • Mice
  • Lung Neoplasms
  • Lung
  • In Situ Hybridization
  • Immunohistochemistry