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Angiogenic growth factors in the pathophysiology of a murine model of acute lung injury.

Publication ,  Journal Article
Karmpaliotis, D; Kosmidou, I; Ingenito, EP; Hong, K; Malhotra, A; Sunday, ME; Haley, KJ
Published in: Am J Physiol Lung Cell Mol Physiol
September 2002

Capillary leakage and alveolar edema are hallmarks of acute lung injury (ALI). Neutrophils and serum macromolecules enter alveoli, promoting inflammation. Vascular endothelial growth factor (VEGF) causes plasma leakage in extrapulmonary vessels. Angiopoietin (Ang)-1 and -4 stabilize vessels, attenuating capillary leakage. We hypothesized that VEGF and Ang-1 and -4 modulate vessel leakage in the lung, contributing to the pathogenesis of ALI. We examined a murine model of lipopolysaccharide (LPS)-induced ALI. C57BL/6 and 129/J mice were studied at baseline and 24, 48, and 96 h after single or multiple doses of aerosolized LPS. Both strains exhibited time- and dose-dependent increases in inflammation and a deterioration of lung mechanics. Bronchoalveolar lavage (BAL) protein levels increased significantly, suggesting capillary leakage. Increased BAL neutrophil and total protein content correlated with time-dependent increased tissue VEGF and decreased Ang-1 and -4 levels, with peak VEGF and minimum Ang-1 and -4 expression after 96 h of LPS challenge. These data suggest that changes in the balance between VEGF and Ang-1 and -4 after LPS exposure may modulate neutrophil influx, protein leakage, and alveolar flooding during early ALI.

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Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

September 2002

Volume

283

Issue

3

Start / End Page

L585 / L595

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Respiratory System
  • Nebulizers and Vaporizers
  • Mice, Inbred Strains
  • Mice, Inbred C57BL
  • Mice
  • Membrane Glycoproteins
  • Lymphokines
  • Lung Diseases
 

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Karmpaliotis, D., Kosmidou, I., Ingenito, E. P., Hong, K., Malhotra, A., Sunday, M. E., & Haley, K. J. (2002). Angiogenic growth factors in the pathophysiology of a murine model of acute lung injury. Am J Physiol Lung Cell Mol Physiol, 283(3), L585–L595. https://doi.org/10.1152/ajplung.00048.2002
Karmpaliotis, Dimitrios, Ioanna Kosmidou, Edward P. Ingenito, Kailin Hong, Atul Malhotra, Mary E. Sunday, and Kathleen J. Haley. “Angiogenic growth factors in the pathophysiology of a murine model of acute lung injury.Am J Physiol Lung Cell Mol Physiol 283, no. 3 (September 2002): L585–95. https://doi.org/10.1152/ajplung.00048.2002.
Karmpaliotis D, Kosmidou I, Ingenito EP, Hong K, Malhotra A, Sunday ME, et al. Angiogenic growth factors in the pathophysiology of a murine model of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2002 Sep;283(3):L585–95.
Karmpaliotis, Dimitrios, et al. “Angiogenic growth factors in the pathophysiology of a murine model of acute lung injury.Am J Physiol Lung Cell Mol Physiol, vol. 283, no. 3, Sept. 2002, pp. L585–95. Pubmed, doi:10.1152/ajplung.00048.2002.
Karmpaliotis D, Kosmidou I, Ingenito EP, Hong K, Malhotra A, Sunday ME, Haley KJ. Angiogenic growth factors in the pathophysiology of a murine model of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2002 Sep;283(3):L585–L595.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

September 2002

Volume

283

Issue

3

Start / End Page

L585 / L595

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factors
  • Vascular Endothelial Growth Factor A
  • Respiratory System
  • Nebulizers and Vaporizers
  • Mice, Inbred Strains
  • Mice, Inbred C57BL
  • Mice
  • Membrane Glycoproteins
  • Lymphokines
  • Lung Diseases