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Tumor necrosis factor induces neuroendocrine differentiation in small cell lung cancer cell lines.

Publication ,  Journal Article
Haley, KJ; Patidar, K; Zhang, F; Emanuel, RL; Sunday, ME
Published in: Am J Physiol
August 1998

We studied tumor necrosis factor (TNF)-alpha as a candidate cytokine to promote neuroendocrine cell differentiation in a nitrosamine-hyperoxia hamster lung injury model. Differential screening identified expression of the genes modulated by TNF-alpha preceding neuroendocrine cell differentiation. Undifferentiated small cell lung carcinoma (SCLC) cell lines NCI-H82 and NCI-H526 were treated with TNF-alpha for up to 2 wk. Both cell lines demonstrated rapid induction of gastrin-releasing peptide (GRP) mRNA; H82 cells also expressed aromatic-L-amino acid decarboxylase mRNA within 5 min after TNF-alpha was added. Nuclear translocation of nuclear factor-kappaB immunostaining occurred with TNF-alpha treatment, suggesting nuclear factor-kappaB involvement in the induction of GRP and/or aromatic-L-amino acid decarboxylase gene expression. We also demonstrated dense core neurosecretory granules and immunostaining for proGRP and neural cell adhesion molecule in H82 cells after 7-14 days of TNF-alpha treatment. We conclude that TNF-alpha can induce phenotypic features of neuroendocrine cell differentiation in SCLC cell lines. Similar effects of TNF-alpha in vivo may contribute to the neuroendocrine cell differentiation/hyperplasia associated with many chronic inflammatory pulmonary diseases.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

August 1998

Volume

275

Issue

2

Start / End Page

L311 / L321

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • RNA, Messenger
  • Nitrosamines
  • Neurosecretory Systems
  • NF-kappa B
  • Lung Neoplasms
  • Lung
  • Hyperoxia
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Haley, K. J., Patidar, K., Zhang, F., Emanuel, R. L., & Sunday, M. E. (1998). Tumor necrosis factor induces neuroendocrine differentiation in small cell lung cancer cell lines. Am J Physiol, 275(2), L311–L321. https://doi.org/10.1152/ajplung.1998.275.2.L311
Haley, K. J., K. Patidar, F. Zhang, R. L. Emanuel, and M. E. Sunday. “Tumor necrosis factor induces neuroendocrine differentiation in small cell lung cancer cell lines.Am J Physiol 275, no. 2 (August 1998): L311–21. https://doi.org/10.1152/ajplung.1998.275.2.L311.
Haley KJ, Patidar K, Zhang F, Emanuel RL, Sunday ME. Tumor necrosis factor induces neuroendocrine differentiation in small cell lung cancer cell lines. Am J Physiol. 1998 Aug;275(2):L311–21.
Haley, K. J., et al. “Tumor necrosis factor induces neuroendocrine differentiation in small cell lung cancer cell lines.Am J Physiol, vol. 275, no. 2, Aug. 1998, pp. L311–21. Pubmed, doi:10.1152/ajplung.1998.275.2.L311.
Haley KJ, Patidar K, Zhang F, Emanuel RL, Sunday ME. Tumor necrosis factor induces neuroendocrine differentiation in small cell lung cancer cell lines. Am J Physiol. 1998 Aug;275(2):L311–L321.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

August 1998

Volume

275

Issue

2

Start / End Page

L311 / L321

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • RNA, Messenger
  • Nitrosamines
  • Neurosecretory Systems
  • NF-kappa B
  • Lung Neoplasms
  • Lung
  • Hyperoxia