Anti-receptor antibody-induced suppression of murine H-Y-specific delayed-type hypersensitivity responses.

Published

Journal Article

A putative anti-H-Y receptor antiserum (ARA) was raised in C57BL/6 male mice against splenic T lymphocytes from syngeneic females immunized against H-Y antigen. When this antiserum is given i.v. to C57BL/6 females it prevents the expression of H-Y-specific delayed-type hypersensitivity (DTH). The suppressive activity in ARA was selectively retained on rabbit anti-mouse immunoglobulin columns, and could be absorbed by H-Y-immune spleen cells from C57BL/6 female mice. The abrogation of H-Y DTH reactivity was at least in part due to the generation of suppressor T cells which are generated by ARA in naive female mice. ARA-generated suppressor cells specifically inhibit the induction phase of DTH responses to the H-Y antigen, having no effect on (4-hydroxy-3-nitrophenyl)acetyl (NP)-specific cutaneous sensitivity responses or on DTH responses to minor histocompatibility antigens. Furthermore, there is a requirement for Igh gene homology between the strain producing the ARA and the strain in which the DTH response is induced. Thus, C57BL/6 ARA given to A.BY (H-2b, Igh-1e) or to B.C-8 (H-2b, Igh-1a) mice was unable to suppress homologous H-Y DTH responses in these strains. However, C57BL/6 ARA induced suppressor cells in B.C-8 mice which were capable of inhibiting H-Y DTH responses when adoptively transferred to C57BL/6 females.

Full Text

Duke Authors

Cited Authors

  • Sunday, ME; Weinberger, JZ; Wolff, S; Dorf, ME

Published Date

  • August 1981

Published In

Volume / Issue

  • 11 / 8

Start / End Page

  • 626 - 631

PubMed ID

  • 6168472

Pubmed Central ID

  • 6168472

International Standard Serial Number (ISSN)

  • 0014-2980

Digital Object Identifier (DOI)

  • 10.1002/eji.1830110807

Language

  • eng

Conference Location

  • Germany