Anti-receptor antibody-induced H-Y-specific delayed-type hypersensitivity responses in nonresponder mice.

Published

Journal Article

The present study examines an antiserum prepared against antigen-reactive T cells that induces murine H-Y-specific delayed-type hypersensitivity (DTH) responses. This anti-H-Y receptor antibody (ARA) was raised in C57BL/6 male mice against splenic T lymphocytes from H-Y immune syngeneic females. Subcutaneous administration of ARA to cyclophosphamide-pretreated C57BL/6 females is able to induce H-Y-specific delayed-type footpad swelling responses. The DTH inducing capacity in ARA was selectively retained on rabbit anti-mouse immunoglobulin columns and was absorbed completely by H-Y immune lymphoid cells from C57BL/6 females. The induction of H-Y DTH reactivity was due at least in part to the activation of H-Y antigen-specific T lymphocytes that could adoptively transfer DTH-like responses to naive female mice. ARA induces DTH responses in strains with the same lgh regions, including selected strains of H-Y nonresponders. Therefore, MHC-linked lr genes do not appear to be as critical when responses are triggered by ARA instead of by antigen. Possible mechanisms for the induction of immune responses by ARA are discussed.

Full Text

Duke Authors

Cited Authors

  • Sunday, ME; Dorf, ME

Published Date

  • April 1, 1983

Published In

Volume / Issue

  • 130 / 4

Start / End Page

  • 1604 - 1609

PubMed ID

  • 6187817

Pubmed Central ID

  • 6187817

International Standard Serial Number (ISSN)

  • 0022-1767

Language

  • eng

Conference Location

  • United States