Syntaxin 1A is transiently expressed in fetal lung mesenchymal cells: potential developmental roles.

Published

Journal Article

Lung development is a complex process in which epithelial-mesenchymal interactions play a key role. A conserved secretory apparatus, the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, is essential for exocytosis in many cell types. Syntaxins, located on the terminal plasma membrane (T-SNAREs), are a critical component of the secretosomal complex involved in vesicular docking, fusion, and exocytosis. We analyzed syntaxin 1A mRNA and protein in fetal rat lung ontogeny, demonstrating peak expression on about day 19 of embryonic development, immediately preceding type II pneumocyte differentiation. Syntaxin 1A is predominantly expressed by lipofibroblasts, which are required for bombesin-like peptide-induced surfactant phospholipid synthesis (choline uptake) by isolated type II cells. In organ cultures, anti-syntaxin 1A antibody HPC-1 blocks choline uptake both at baseline and when induced by bombesin-like peptide or dexamethasone. HPC-1 also promotes thymidine uptake in parallel in a dose-dependent fashion. These observations indicate a potential role for syntaxin 1A during fetal lung development, possibly through involvement in secretion of mesenchymal cell-derived factors that induce terminal type II cell differentiation.

Full Text

Duke Authors

Cited Authors

  • Brimhall, BB; Sikorski, KA; Torday, J; Shahsafaei, A; Haley, KJ; Sunday, ME

Published Date

  • August 1999

Published In

Volume / Issue

  • 277 / 2

Start / End Page

  • L401 - L411

PubMed ID

  • 10444535

Pubmed Central ID

  • 10444535

International Standard Serial Number (ISSN)

  • 0002-9513

Digital Object Identifier (DOI)

  • 10.1152/ajplung.1999.277.2.L401

Language

  • eng

Conference Location

  • United States