A single-tube screen for Salmonella and Shigella.

Journal Article

Salmonella and Shigella species are routinely sought in stool specimens submitted for culture. It is a common practice to screen lactose-negative colonies by using triple sugar iron agar, lysine iron agar, and Christensen urea agar to determine if further identification is necessary. We designed and evaluated a novel combination of media, which are layered in a single tube, for screening isolates suspected to possibly represent Salmonella or Shigella. We tested this media combination with 106 Salmonella, 56 Shigella, and 56 other gram-negative bacilli. All Salmonella and Shigella isolates tested were appropriately characterized as possible Salmonella or Shigella by using an algorithm developed for use with this media combination. Similarly, 53 (95%) of 56 other gram-negative bacilli were appropriately screened as non -Salmonella and non -Shigella isolates. This unique media combination provides the most important biochemical reactions needed to screen for Salmonella and Shigella in a single-tube format, which decreases labor by two thirds (ie, 1 tube is inoculated vs 3).

Full Text

Duke Authors

Cited Authors

  • Procop, GW; Wallace, JD; Tuohy, MJ; Lasalvia, MM; Addison, RM; Reller, LB

Published Date

  • August 2008

Published In

Volume / Issue

  • 130 / 2

Start / End Page

  • 284 - 289

PubMed ID

  • 18628099

International Standard Serial Number (ISSN)

  • 0002-9173

Digital Object Identifier (DOI)

  • 10.1309/MTDBAXHDPKAL6GF5

Language

  • eng

Conference Location

  • England