Detection of Pneumocystis jiroveci in respiratory specimens by four staining methods.

Journal Article (Journal Article)

We examined four staining methods on replicate smears of 313 respiratory specimens submitted for Pneumocystis jiroveci examination. The sensitivity and specificity of Calcofluor white stain (CW) were 73.8 and 99.6%, respectively. The sensitivity and specificity of Grocott-Gomori methenamine silver stain (GMS) were 79.4 and 99.2%, respectively. The sensitivity and specificity of Diff-Quik stain were 49.2 and 99.6%, respectively. The sensitivity and specificity of Merifluor Pneumocystis stain were 90.8 and 81.9%, respectively. Only CW and GMS had positive and negative predictive values of >90%.

Full Text

Duke Authors

Cited Authors

  • Procop, GW; Haddad, S; Quinn, J; Wilson, ML; Henshaw, NG; Reller, LB; Artymyshyn, RL; Katanik, MT; Weinstein, MP

Published Date

  • July 1, 2004

Published In

Volume / Issue

  • 42 / 7

Start / End Page

  • 3333 - 3335

PubMed ID

  • 15243109

Pubmed Central ID

  • PMC446244

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/JCM.42.7.3333-3335.2004

Language

  • eng

Conference Location

  • United States