Selection of strains for quality assessment of the disk induction method for detection of inducible clindamycin resistance in Staphylococci: a CLSI collaborative study.

Journal Article (Journal Article;Multicenter Study)

A nine-laboratory collaborative study was conducted to select positive and negative quality assessment control strains for the detection of inducible clindamycin resistance in staphylococci. Four strains of Staphylococcus aureus were tested as unknowns on 10 different days in each laboratory using the recently recommended CLSI (formerly NCCLS) disk diffusion method and the inoculum purity control method. Strains contained either macrolide-lincosamide-streptogramin B (MLSB) resistance genes encoded by erm(A) or erm(C) or a macrolide resistance efflux pump encoded by msr(A). Based upon the results of this study, strain UT 32 (now designated ATCC strain BAA-977) containing erm(A) is recommended as the positive control organism for inducible clindamycin resistance. Strain UT 25 (now designated ATCC BAA-976), which harbors the efflux pump encoded by msr(A), is recommended as the negative control organism.

Full Text

Duke Authors

Cited Authors

  • Zelazny, AM; Ferraro, MJ; Glennen, A; Hindler, JF; Mann, LM; Munro, S; Murray, PR; Reller, LB; Tenover, FC; Jorgensen, JH

Published Date

  • June 2005

Published In

Volume / Issue

  • 43 / 6

Start / End Page

  • 2613 - 2615

PubMed ID

  • 15956373

Pubmed Central ID

  • PMC1151953

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/JCM.43.6.2613-2615.2005


  • eng

Conference Location

  • United States