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Multicenter evaluation of the new Vitek 2 Neisseria-Haemophilus identification card.

Publication ,  Journal Article
Rennie, RP; Brosnikoff, C; Shokoples, S; Reller, LB; Mirrett, S; Janda, W; Ristow, K; Krilcich, A
Published in: J Clin Microbiol
August 2008

The new Neisseria-Haemophilus identification (NH) card for Vitek 2 was compared with 16S rRNA gene sequencing (16S) as the reference method for accurate identification of Neisseria spp., Haemophilus spp., and other fastidious gram-negative bacteria. Testing was performed on the Vitek 2 XL system with modified software at three clinical trial laboratories. Reproducibility was determined with nine ATCC quality control strains tested 20 times over a minimum of 10 days at all three sites. A challenge set of 30 strains with known identifications and 371 recent fresh and frozen clinical isolates were also tested. Expected positive and negative biochemical reactions were also evaluated for substrate reproducibility. All microorganisms were tested on the NH card, and all clinical and stock isolates were saved for 16S testing. All reproducibility tests yielded expected results within a 95% confidence interval. For challenge microorganisms, there was 98% overall correct identification, including 8% low discrimination, 2% incorrect identification, and 0% unidentified. For clinical strains, there was 96.5% overall correct identification, including 10.2% low discrimination, 2.7% incorrect identification, and 0.8% unidentified. The 2.7% (10/371) of clinical isolates that gave an incorrect identification consisted of 7 isolates correct to genus and 3 strains incorrect to genus. There were an additional 27 strains (primarily Neisseria species) for which the 16S identification result was different from the NH card result. These were all unclaimed species by the system. The new NH card met all performance criteria within a 95% confidence interval compared to identification of clinical isolates by 16S.

Duke Scholars

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

August 2008

Volume

46

Issue

8

Start / End Page

2681 / 2685

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Sensitivity and Specificity
  • Reproducibility of Results
  • RNA, Ribosomal, 16S
  • Neisseria
  • Microbiology
  • Humans
  • Haemophilus
  • DNA, Bacterial
  • Bacterial Typing Techniques
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Rennie, R. P., Brosnikoff, C., Shokoples, S., Reller, L. B., Mirrett, S., Janda, W., … Krilcich, A. (2008). Multicenter evaluation of the new Vitek 2 Neisseria-Haemophilus identification card. J Clin Microbiol, 46(8), 2681–2685. https://doi.org/10.1128/JCM.00449-08
Rennie, Robert P., Cheryl Brosnikoff, Sandy Shokoples, L Barth Reller, Stanley Mirrett, William Janda, Kathy Ristow, and Ann Krilcich. “Multicenter evaluation of the new Vitek 2 Neisseria-Haemophilus identification card.J Clin Microbiol 46, no. 8 (August 2008): 2681–85. https://doi.org/10.1128/JCM.00449-08.
Rennie RP, Brosnikoff C, Shokoples S, Reller LB, Mirrett S, Janda W, et al. Multicenter evaluation of the new Vitek 2 Neisseria-Haemophilus identification card. J Clin Microbiol. 2008 Aug;46(8):2681–5.
Rennie, Robert P., et al. “Multicenter evaluation of the new Vitek 2 Neisseria-Haemophilus identification card.J Clin Microbiol, vol. 46, no. 8, Aug. 2008, pp. 2681–85. Pubmed, doi:10.1128/JCM.00449-08.
Rennie RP, Brosnikoff C, Shokoples S, Reller LB, Mirrett S, Janda W, Ristow K, Krilcich A. Multicenter evaluation of the new Vitek 2 Neisseria-Haemophilus identification card. J Clin Microbiol. 2008 Aug;46(8):2681–2685.

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

August 2008

Volume

46

Issue

8

Start / End Page

2681 / 2685

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Sensitivity and Specificity
  • Reproducibility of Results
  • RNA, Ribosomal, 16S
  • Neisseria
  • Microbiology
  • Humans
  • Haemophilus
  • DNA, Bacterial
  • Bacterial Typing Techniques