Cloning of human and mouse brain cDNAs coding for S1, the second member of the mammalian elongation factor-1 alpha gene family: analysis of a possible evolutionary pathway.

Journal Article

We previously reported the cloning of a rat S1 cDNA whose deduced amino acid sequence shares high similarity (92%) with that of mammalian elongation factor-1 alpha (EF-1 alpha), a protein involved in the binding of aminoacyl-tRNA to the ribosome during peptide synthesis. We report here the isolation of a full-length cDNA from a mouse brain library and a partial-length cDNA from a human hippocampus library which share extensive sequence similarity to rat S1 cDNA. We show that, as with mammalian EF-1 alpha S, the predicted primary amino acid sequences of rat, mouse, and human S1 are almost identical, except for one conservative substitution. These results indicate that mouse and man contain a second member of the EF-1 alpha gene family, the S1 gene. They also suggest that our result obtained in rat may be extrapolated to mouse and man.

Full Text

Duke Authors

Cited Authors

  • Lee, S; Ann, DK; Wang, E

Published Date

  • September 30, 1994

Published In

Volume / Issue

  • 203 / 3

Start / End Page

  • 1371 - 1377

PubMed ID

  • 7945283

International Standard Serial Number (ISSN)

  • 0006-291X

Language

  • eng

Conference Location

  • United States