Liposome-mediated conformation transition of DNA detected by molecular probe: methyl green.

Published

Journal Article

Recent studies have focused on the structural features of DNA-lipid assemblies. In this paper, we take methyl green (MG) as a probe molecule to detect the conformational change of DNA molecule induced by dimethyldioctadecylammonium bromide (DDAB) liposomes before the condensation process of DNA begins. DDAB-induced DNA topology changes were investigated by cyclic voltammetry (CV), circular dichroism (CD) and UV-VIS spectrometry. We find that upon binding to DNA, positively charged liposomes induce a conformational transition of DNA molecules from the native B-form to the C motif. Conformational transition in DNA results in the binding modes of MG to DNA, changing and being isolated from DNA to the solution. More stable complexes are formed between DNA and DDAB. That is also proved by the melting study of DNA.

Full Text

Duke Authors

Cited Authors

  • Huang, W; Zhang, Z; Han, X; Tang, J; Wang, J; Dong, S; Wang, E

Published Date

  • April 2003

Published In

Volume / Issue

  • 59 / 1-2

Start / End Page

  • 21 - 27

PubMed ID

  • 12699815

Pubmed Central ID

  • 12699815

International Standard Serial Number (ISSN)

  • 1567-5394

Language

  • eng

Conference Location

  • Netherlands