Liposome-mediated conformation transition of DNA detected by molecular probe: methyl green.
Journal Article (Journal Article)
Recent studies have focused on the structural features of DNA-lipid assemblies. In this paper, we take methyl green (MG) as a probe molecule to detect the conformational change of DNA molecule induced by dimethyldioctadecylammonium bromide (DDAB) liposomes before the condensation process of DNA begins. DDAB-induced DNA topology changes were investigated by cyclic voltammetry (CV), circular dichroism (CD) and UV-VIS spectrometry. We find that upon binding to DNA, positively charged liposomes induce a conformational transition of DNA molecules from the native B-form to the C motif. Conformational transition in DNA results in the binding modes of MG to DNA, changing and being isolated from DNA to the solution. More stable complexes are formed between DNA and DDAB. That is also proved by the melting study of DNA.
Full Text
Duke Authors
Cited Authors
- Huang, W; Zhang, Z; Han, X; Tang, J; Wang, J; Dong, S; Wang, E
Published Date
- April 2003
Published In
Volume / Issue
- 59 / 1-2
Start / End Page
- 21 - 27
PubMed ID
- 12699815
International Standard Serial Number (ISSN)
- 1567-5394
Digital Object Identifier (DOI)
- 10.1016/s1567-5394(02)00185-8
Language
- eng
Conference Location
- Netherlands