Concentration-dependent behavior of nisin interaction with supported bilayer lipid membrane.

Journal Article (Journal Article)

Nisin is a positively charged antibacterial peptide that binds to the negatively charged membranes of gram-positive bacteria. The initial interaction of the peptide with the model membrane of negatively charged DPPG (dipalmitoylphosphatidylglycerol) was studied by cyclic voltammetry and a.c. impedance spectroscopy. Nisin could induce pores in the supported bilayer lipid membrane, thus, it led to the marker ions Fe(CN)(6)(3-/4-) crossing the lipid membrane and giving the redox reaction on the glassy carbon electrode (GCE). Experimental results suggested that the pore formation on supported bilayer lipid membrane was dependent on the concentration of nisin and it included three main concentration stages: low, middling, high concentration.

Full Text

Duke Authors

Cited Authors

  • Huang, W; Zhang, Z; Han, X; Wang, J; Tang, J; Dong, S; Wang, E

Published Date

  • November 6, 2002

Published In

Volume / Issue

  • 99 / 3

Start / End Page

  • 271 - 279

PubMed ID

  • 12408941

International Standard Serial Number (ISSN)

  • 0301-4622

Digital Object Identifier (DOI)

  • 10.1016/s0301-4622(02)00227-2


  • eng

Conference Location

  • Netherlands