pH-dependent conformational changes of ferricytochrome c induced by electrode surface microstructure.

Journal Article (Journal Article)

pH-dependent processes of bovine heart ferricytochrome c have been investigated by electronic absorption and circular dichroism (CD) spectra at functionalized single-wall carbon nanotubes (SWNTs) modified glass carbon electrode (SWNTs/GCE) using a long optical path thin layer cell. These methods enabled the pH-dependent conformational changes arising from the heme structure change to be monitored. The spectra obtained at functionalized SWNTs/GCE reflect electrode surface microstructure-dependent changes for pH-induced protein conformation, pK(a) of alkaline transition and structural microenvironment of the ferricytochrome c heme. pH-dependent conformational distribution curves of ferricytochrome c obtained by analysis of in situ CD spectra using singular value decomposition least square (SVDLS) method show that the functionalized SWNTs can retain native conformational stability of ferricytochrome c during alkaline transition.

Full Text

Duke Authors

Cited Authors

  • Jiang, X; Qu, X; Zhang, L; Zhang, Z; Jiang, J; Wang, E; Dong, S

Published Date

  • August 1, 2004

Published In

Volume / Issue

  • 110 / 3

Start / End Page

  • 203 - 211

PubMed ID

  • 15228956

International Standard Serial Number (ISSN)

  • 0301-4622

Digital Object Identifier (DOI)

  • 10.1016/j.bpc.2004.02.004


  • eng

Conference Location

  • Netherlands