Conformational transition of DNA induced by cationic lipid vesicle in acidic solution: spectroscopy investigation.
Published
Journal Article
The conformational transition of DNA induced by the interaction between DNA and a cationic lipid vesicle, didodecyldimethylammonium bromide (DDAB), had been investigated by circular dichroism (CD) and UV spectroscopy methods. We used singular value decomposition least squares method (SVDLS) to analyze the experimental CD spectra. Although pH value influenced the conformation of DNA in solution, the results showed that upon binding to double helical DNA, positively charged liposomes induced a conformational transition of DNA molecules from the native B-form to more compact conformations. At the same time, no obvious conformational changes occurred at single-strand DNA (ssDNA). While the cationic lipid vesicles and double-strand DNA (dsDNA) were mixed at a high molar ratio of DDAB vesicles to dsDNA, the conformation of dsDNA transformed from the B-form to the C-form resulting in an increase in duplex stability (DeltaT(m)=8+/-0.4 degrees C). An increasing in T(m) was also observed while the cationic lipid vesicles interacted with ssDNA.
Full Text
Duke Authors
Cited Authors
- Zhang, Z; Huang, W; Tang, J; Wang, E; Dong, S
Published Date
- May 23, 2002
Published In
Volume / Issue
- 97 / 1
Start / End Page
- 7 - 16
PubMed ID
- 12052491
Pubmed Central ID
- 12052491
International Standard Serial Number (ISSN)
- 0301-4622
Digital Object Identifier (DOI)
- 10.1016/s0301-4622(02)00006-6
Language
- eng
Conference Location
- Netherlands