Advantages of mRNA amplification for microarray analysis.

Published

Journal Article

Expanding applications of cDNA microarrays such as fine needle aspiration biopsy and laser capture microdissection necessitate the ability to perform arrays with minute starting amounts of RNA. While methods for amplifying RNA have been advocated, the fidelity of array results using amplified material has not been fully validated. Here we demonstrate preserved fidelity in arrays using one or two rounds of mRNA amplification, validated by downstream real-time quantitative PCR. In addition, the quality of the array data was superior to that obtained using total RNA. Based on these results, we recommend routine mRNA amplification for all cDNA microarray-based analysis of gene expression.

Full Text

Duke Authors

Cited Authors

  • Feldman, AL; Costouros, NG; Wang, E; Qian, M; Marincola, FM; Alexander, HR; Libutti, SK

Published Date

  • October 2002

Published In

Volume / Issue

  • 33 / 4

Start / End Page

  • 906 - 914

PubMed ID

  • 12398200

Pubmed Central ID

  • 12398200

Electronic International Standard Serial Number (EISSN)

  • 1940-9818

International Standard Serial Number (ISSN)

  • 0736-6205

Digital Object Identifier (DOI)

  • 10.2144/02334mt04

Language

  • eng