Venezuelan equine encephalitis virus transmission and effect on pathogenesis.

Published

Journal Article

Quantifying the dose of an arbovirus transmitted by mosquitoes is essential for designing pathogenesis studies simulating natural infection of vertebrates. Titration of saliva collected in vitro from infected mosquitoes may not accurately estimate titers transmitted during blood feeding, and infection by needle injection may affect vertebrate pathogenesis. We compared the amount of Venezuelan equine encephalitis virus collected from the saliva of Aedes taeniorhynchus to the amount injected into a mouse during blood feeding. Less virus was transmitted by mosquitoes in vivo (geometric mean 11 PFU) than was found for comparable times of salivation in vitro (mean saliva titer 74 PFU). We also observed slightly lower early and late viremia titers in mice that were needle injected with 8 PFU, which represents the low end of the in vivo transmission range. No differences in survival were detected, regardless of the dose or infection route.

Full Text

Duke Authors

Cited Authors

  • Smith, DR; Aguilar, PV; Coffey, LL; Gromowski, GD; Wang, E; Weaver, SC

Published Date

  • August 2006

Published In

Volume / Issue

  • 12 / 8

Start / End Page

  • 1190 - 1196

PubMed ID

  • 16965696

Pubmed Central ID

  • 16965696

International Standard Serial Number (ISSN)

  • 1080-6040

Digital Object Identifier (DOI)

  • 10.3201/eid1708.050841

Language

  • eng

Conference Location

  • United States