Fumonisin B1 consumption by rats causes reversible, dose-dependent increases in urinary sphinganine and sphingosine.

Journal Article (Journal Article)

Fumonisin B1 (FB1) is a frequently encountered mycotoxin that inhibits ceramide synthase, the enzyme that acylates sphinganine, sphingosine and other "sphingoid" bases. Exposure of rats, rabbits, pigs and nonhuman primates to fumonisin-contaminated feed elevates sphingoid base amounts in urine; therefore, this study examined the time course and reversibility of these changes. When an AIN-76 diet supplemented with >/=5 microg FB1/g was fed to male Sprague-Dawley rats, there was a significant increase in sphinganine (ca. 50-fold in urine from rats fed 50 microg FB1/g diet) and smaller changes in sphingosine within 5 to 7 d, compared to rats fed the same diet without FB1. No change occurred in sphingoid bases upon feeding 1 microg FB1/g for up to 60 d. When rats were fed FB1 (10 microg FB1/g diet for 10 d), then changed to the same diet minus FB1, urinary sphingoid bases returned to normal within 10 d. However, if the rats were fed 10 microg FB1/g for 10 d, then changed to 1 microg FB1/g, the amounts of sphingoid bases in urine were the same as for rats that were continuously fed 10 microg FB1/g. These results establish that consumption of FB1 causes dose-dependent and reversible elevations in the amounts of urinary sphingoid bases. The finding that 1 microg FB1/g (which does not, alone, alter urinary sphingoid bases) will sustain the elevation caused by previous exposure to 10 microg FB1/g raises the possibility that even low levels of fumonisins could be deleterious when an animal is occasionally exposed to higher amounts.

Full Text

Duke Authors

Cited Authors

  • Wang, E; Riley, RT; Meredith, FI; Merrill, AH

Published Date

  • January 1999

Published In

Volume / Issue

  • 129 / 1

Start / End Page

  • 214 - 220

PubMed ID

  • 9915902

International Standard Serial Number (ISSN)

  • 0022-3166

Digital Object Identifier (DOI)

  • 10.1093/jn/129.1.214


  • eng

Conference Location

  • United States