A modified procedure for fast purification of T7 RNA polymerase.

Journal Article (Journal Article)

The in vitro T7 transcription system allows one to synthesize biochemical amounts of RNA molecules functionally equivalent or similar to those transcripts normally existing at extremely low levels in vivo. In this study we described a modified method for efficient large-scale preparation of pure T7 RNA polymerase free of RNase activity from the recombinant Escherichia coli strain BL21/pAR1219 (4). The procedure, which used preparative column chromatography on DEAE-Sepharose CL-6B and Blue 3GA, was shown to be simple, rapid, and cost effective in comparison with other methods reported previously.

Full Text

Duke Authors

Cited Authors

  • Li, Y; Wang, E; Wang, Y

Published Date

  • July 1999

Published In

Volume / Issue

  • 16 / 2

Start / End Page

  • 355 - 358

PubMed ID

  • 10419832

International Standard Serial Number (ISSN)

  • 1046-5928

Digital Object Identifier (DOI)

  • 10.1006/prep.1999.1083


  • eng

Conference Location

  • United States