The differential expression of statin in the nuclei of human colonic crypts adjacent to a cancer: an immunohistochemical study.

Journal Article

Statin, a non-proliferation-specific nuclear antigen, was used here to assess the colonic crypt kinetics of the mucosa bordering a human colon cancer. Mucosal strips adjacent to a colon cancer obtained from operative specimens were immediately cut into five one cm segments and stored in liquid nitrogen. An immunohistological technique using the statin antibody as a nuclear marker was used to determine the labelling indices of the non-cycling compartment at the varying distances. Optical density measurements of the nuclear reaction product served to objectively identify the statin-positive nucleus. The results indicate that there is a statistically significant reduction (P < 0.0001) in the statin-positive labelling index in the entire crypt length for a distance of three cms. The division of the entire crypt into four levels (A, B, C and D) demonstrates that this effect is principally due to the upward extension of the statin-negative cell mass into levels B and C with a corresponding decrease in the labelling index of the statin-positive nuclei in these levels. The in vivo expression of nuclear statin demonstrates its usefulness in accurately determining the size of the non-proliferative compartment in the human colonic crypt adjacent to a colon cancer.

Full Text

Duke Authors

Cited Authors

  • Mitmaker, B; Bayer, I; Baytner, S; Gordon, PH; Wang, E

Published Date

  • 1993

Published In

Volume / Issue

  • 37 / 1

Start / End Page

  • 43 - 51

PubMed ID

  • 7682866

International Standard Serial Number (ISSN)

  • 1121-760X

Language

  • eng

Conference Location

  • Italy