Identification of the segments of the mouse transferrin receptor 1 required for mouse mammary tumor virus infection.

Journal Article

Most enveloped viruses enter cells through binding of virion surface envelope proteins to receptors found on the plasma membrane of the cell. The beta retrovirus mouse mammary tumor virus (MMTV) uses transferrin receptor 1 (TfR1) to enter cells in a pH-dependent mechanism, probably co-trafficking with TfR1 to an acidic compartment where virus entry occurs. We have shown here that, although mouse and rat TfR1 function as entry receptors, cat, dog, hamster, or human TfR1s do not support MMTV infection. We also demonstrated that MMTV entry is independent of transferrin, iron, and the TfR1 cofactor hereditary hematochromatosis HFE protein. Using chimeric mouse/human hybrid TfR1 constructs, we determined the site of interaction with MMTV and found that it maps to two segments physically disparate from the TfR and HFE binding sites. Thus, MMTV has apparently evolved to enter cells independently of the iron status of the host.

Full Text

Duke Authors

Cited Authors

  • Wang, E; Albritton, L; Ross, SR

Published Date

  • April 14, 2006

Published In

Volume / Issue

  • 281 / 15

Start / End Page

  • 10243 - 10249

PubMed ID

  • 16481319

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.M511572200

Language

  • eng

Conference Location

  • United States