Characterization and property of DNA incorporated bilayer lipid membranes.

Journal Article (Journal Article)

Calf-thymus DNA-incorporated bilayer lipid membranes supported on a glassy carbon (GC) electrode was prepared by making layers of phosphatidylcholine dimyristoyl (DMPC) on GC electrode. DNA in the BLM was characterized by cyclic voltammetry, IR and AFM, and lipid layers formed on the GC electrode were demonstrated to be a bilayer lipid membrane by electrochemical impedance experiment. In IR and AFM experiments the findings indicated that DNA was incorporated into BLM. The ion channel of bilayer lipid membranes incorporated was studied. The result showed that the ion channel was opened in the presence of the stimulus quinacrine. In the absence of quinacrine the channel was switched. The process can repeat itself many times. The impedance spectroscopy measurements demonstrate that the stimulus quinacrine opens the channel for permeation of marker ion. The mechanism of forming an ion channel was investigated.

Full Text

Duke Authors

Cited Authors

  • Tong, Y; Han, X; Song, Y; Jiang, J; Wang, E

Published Date

  • August 1, 2003

Published In

Volume / Issue

  • 105 / 1

Start / End Page

  • 1 - 9

PubMed ID

  • 12932574

International Standard Serial Number (ISSN)

  • 0301-4622

Digital Object Identifier (DOI)

  • 10.1016/s0301-4622(02)00336-8


  • eng

Conference Location

  • Netherlands