Determination of diphenhydramine by capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection.

Journal Article (Journal Article)

Tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection in a capillary electrophoresis separation system was used for the determination of diphenhydramine. In this study, platinum disk electrode (300 mum in diameter) was used as a working electrode and the influence of applied potential and buffer conditions were investigated. Under optimal conditions: 1.2 V applied potential, pH 8.50, 15 kV separation voltage and 10 mmoll(-1) running buffer, the calibration curve of diphenhydramine was linear over the range of 4x10(-8) to 1x10(-5) moll(-1). This technique gave satisfactory precision, and relative standard deviations of migration times and chemiluminescence peak intensities were less than 1 and 6%, respectively. The technique was applied to animal studies for determination of diphenhydramine extracted from rabbit plasma and urine samples, and the extraction efficiency were between 92 and 98.5%.

Full Text

Duke Authors

Cited Authors

  • Liu, J; Cao, W; Yang, X; Wang, E

Published Date

  • March 1, 2003

Published In

Volume / Issue

  • 59 / 3

Start / End Page

  • 453 - 459

PubMed ID

  • 18968929

Electronic International Standard Serial Number (EISSN)

  • 1873-3573

Digital Object Identifier (DOI)

  • 10.1016/s0039-9140(02)00535-0


  • eng

Conference Location

  • Netherlands