A leukocyte lipid up-regulates the avidity of lymphocyte function-associated antigen-1.

Published

Journal Article

An acidic lipid termed leukocyte adhesion lipid (LAL) was isolated from PMA stimulated lymphoid and myeloid cell lines HL60, Jurkat, K562 and U937 but not from unstimulated cells or PMA treated Cos7 cells. LAL treated peripheral blood leukocytes (PBL) adhered strongly to IL-1 beta activated human umbilical vein endothelial cells (HUVEC), and the interaction could be inhibited by antibodies to intercellular adhesion molecule (ICAM-1) or lymphocyte function-associated antigen-1 (LFA-1). Leukocytes treated with LAL maintained the high avidity state of LFA-1 for at least 1 hr whereas the avidity of LFA-1 in PMA treated cells declined after 30 min. LAL was stable to heat (100 degrees C, 10 min), alkaline phosphatase and proteinase K treatments. Chemical analysis suggested that LAL contained unsaturated lipids. Our findings provide evidence for the involvement of lipids in LFA-1 activation.

Full Text

Duke Authors

Cited Authors

  • Lee, DH; MacIntyre, JP; Wang, E; Hudson, DJ; Ishaque, A; Conant, JA; Pope, BL; Lau, CY

Published Date

  • February 28, 1994

Published In

Volume / Issue

  • 199 / 1

Start / End Page

  • 319 - 326

PubMed ID

  • 7907214

Pubmed Central ID

  • 7907214

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1994.1231

Language

  • eng

Conference Location

  • United States