Two-photon molecular excitation imaging of Ca2+ transients in Langendorff-perfused mouse hearts.
The ability to image calcium signals at subcellular levels within the intact depolarizing heart could provide valuable information toward a more integrated understanding of cardiac function. Accordingly, a system combining two-photon excitation with laser-scanning microscopy was developed to monitor electrically evoked [Ca(2+)](i) transients in individual cardiomyocytes within noncontracting Langendorff-perfused mouse hearts. [Ca(2+)](i) transients were recorded at depths
Rubart, M; Wang, E; Dunn, KW; Field, LJ
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