Scanning tunnelling microscopy observation of cytochrome-c denaturation induced by bromopyrogal red on highly oriented pyrolytic graphite.

Journal Article (Journal Article)

The denaturation of cytochrome-c (cyt-c) induced by bromopyrogal red (BPR) was studied by scanning tunnelling microscopy (STM) on the electrochemically pretreated highly oriented pyrolytic graphite (HOPG) surface. STM images reveal that denatured cyt-c molecules exist in variable states including aggregates, globular compact, partially unfolded and combined with BPR molecule. The apparently low image contrast of denatured cyt-c observed in this experiment comparing to that of native cyt-c molecules, and the relative low image contrast of the unfolded part comparing with the compact globular part, are ascribed to the unfavourable tunnelling paths for the conformational variations of denatured cyt-c molecules.

Full Text

Duke Authors

Cited Authors

  • Zhang, B; Zhu, Y; Dong, S; Wang, E

Published Date

  • October 1997

Published In

Volume / Issue

  • 21 / 3

Start / End Page

  • 251 - 261

PubMed ID

  • 9352371

International Standard Serial Number (ISSN)

  • 0141-8130

Digital Object Identifier (DOI)

  • 10.1016/s0141-8130(97)00073-1


  • eng

Conference Location

  • Netherlands