Kinetic study of paracetamol on prolidase activity in erythrocytes by capillary electrophoresis with Ru(bpy)(3) (2+) electrochemiluminescence detection.
Journal Article (Journal Article)
We explored the CE with (bpy) (2+) (3) electrochemiluminescence detection for the kinetic study of drug-enzyme interaction. Effects of four nonsteroidal anti-inflammatory drugs including aspirin, paracetamol, sodium salicylate and phenacetin on prolidase (PLD) activity in erythrocytes were investigated. Aspirin enhanced PLD activity whereas the other three had inhibiting effects. This may reveal their different effects on the collagen biosynthesis and catabolism that influence tumor invasiveness. Kinetic study of paracetamol on PLD showed that the value of Michaelis constant K(m) for PLD was 1.23 mM. The mechanism of PLD inhibition by paracetamol is noncompetitive inhibition, and the inhibitor constant K(i) value obtained in our research was 9.73 x 10(3) microg/L.
Full Text
Duke Authors
Cited Authors
- Yuan, J; Wei, H; Jin, W; Yang, X; Wang, E
Published Date
- October 2006
Published In
Volume / Issue
- 27 / 20
Start / End Page
- 4047 - 4051
PubMed ID
- 16991207
International Standard Serial Number (ISSN)
- 0173-0835
Digital Object Identifier (DOI)
- 10.1002/elps.200600197
Language
- eng
Conference Location
- Germany