Differential expression of S1 and elongation factor-1 alpha during rat development.

Published

Journal Article

Elongation factor-1 alpha (EF-1 alpha) is a highly conserved protein functioning in peptide elongation during translation. A cDNA, S1, was isolated; its deduced amino acid sequence shares high similarity with mammalian EF-1 alpha s (92%). While EF-1 alpha is present in all tissues, S1 mRNA can only be detected in brain, heart, and muscle. We report here that the retropseudogene phenomenon is attributable to EF-1 alpha and not S1, the latter being represented by a single copy in the rat genome. The S1 steady-state mRNA levels are consistently higher than EF-1 alpha in S1-positive tissues. S1 mRNA can only be detected late during brain, heart, and muscle development in vivo and increases to a plateau in early postnatal life. In a cultured muscle system, S1 expression is dependent upon the formation of myotubes, although the accumulation of S1 mRNA is significantly lower than that observed in adult skeletal muscle. EF-1 alpha mRNA levels are down-regulated during brain, heart, and muscle development, but stay relatively steady in liver. We show here that EF-1 alpha and S1 are differentially expressed during rat development and that the activation of S1 gene expression is subsequent to the terminal differentiation process in brain, heart, and muscle.

Full Text

Duke Authors

Cited Authors

  • Lee, S; Wolfraim, LA; Wang, E

Published Date

  • November 15, 1993

Published In

Volume / Issue

  • 268 / 32

Start / End Page

  • 24453 - 24459

PubMed ID

  • 8226996

Pubmed Central ID

  • 8226996

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States