Microfluidic chip with electrochemiluminescence detection using 2-(2-aminoethyl)-1-methylpyrrolidine labeling.

Journal Article (Journal Article)

A tertiary amine derivative, 2-(2-aminoethyl)-1-methylpyrrolidine (AEMP) was successfully developed as electrochemiluminescence (ECL) probe within microfluidic chip using ECL detection in this paper. The system was characterized by the interaction between biotin and avidin. In principle, tertiary amine derivatives containing active group can be used as a potential alternative of traditional tris(2,2'-bipyridine)ruthenium(II) [Ru(bpy)3(2+)] label. Firstly, The ECL efficiency of AEMP was characterized via comparing with that of two coreactants enhancing Ru(bpy)3(2+) ECL, TPA and proline. At same condition, AEMP has a similar ECL efficiency to TPA, and much higher than proline. After AEMP reacted with NHS-LC-biotin (succinimidyl-6-(biotinamido) hexanoate), the products and their ECL were analyzed by directly injecting it in the microfluidic chip. A 4.5 cm microchannel was used to separate the mixture of AEMP and biotinylated AEMP. The present works indicated that AEMP has a good reactivity to the analytes containing carboxyl group with a similar ECL efficiency to TPA. Under optimal condition, the detection limits (based on 3 S/N) of AEMP was 2.7 microM. The system was also validated by the reaction between biotin and avidin. The calculated binding ratio between avidin and biotin based on the present method was 4.4.

Full Text

Duke Authors

Cited Authors

  • Yin, X-B; Du, Y; Yang, X; Wang, E

Published Date

  • October 14, 2005

Published In

Volume / Issue

  • 1091 / 1-2

Start / End Page

  • 158 - 162

PubMed ID

  • 16395805

International Standard Serial Number (ISSN)

  • 0021-9673

Digital Object Identifier (DOI)

  • 10.1016/j.chroma.2005.07.046


  • eng

Conference Location

  • Netherlands