Kinetic study of collagen fixation with polyepoxy fixatives.

Journal Article (Journal Article)

A new biomaterial has been developed by fixing native collagen with a polyepoxy compound (PC) fixative. In this study, bovine internal thoracic arteries were fixed with PC under various conditions to help understand the kinetics of the collagen-PC reactions and optimize the fixation process. At predetermined time intervals, small samples were cut from the arteries to determine the quantities of the remaining unreacted amino acids in the collagen. Temperature, concentration, and solution pH were among the key parameters studied. The overall fixation rate was found to be reaction-rate controlled, as the rate of fixation was relatively slow compared with the rate of diffusion of PC. As might be expected, the reaction rate was favored by a higher temperature, concentration, and solution pH. A kinetic model, with a 2.5th reaction order with respect to the reactive functional groups of collagen and a first order with respect to PC, was developed that gave a good fit to the experimental data. Based on this model, the degree of fixation, X, as a function of time, t, is given by (1 - X)-1.5 = 1 + Kt, where K is a constant related to the initial concentrations and the reaction rate constant.

Full Text

Duke Authors

Cited Authors

  • Tu, R; Lu, CL; Thyagarajan, K; Wang, E; Nguyen, H; Shen, S; Hata, C; Quijano, RC

Published Date

  • January 1, 1993

Published In

Volume / Issue

  • 27 / 1

Start / End Page

  • 3 - 9

PubMed ID

  • 8420998

International Standard Serial Number (ISSN)

  • 0021-9304

Digital Object Identifier (DOI)

  • 10.1002/jbm.820270103


  • eng

Conference Location

  • United States