Direct electrochemical detection of glucose in human plasma on capillary electrophoresis microchips.

Published

Journal Article

We developed an electrochemical detector on a hybrid chip for the determination of glucose in human plasma. The microchip system described in this paper consists of a poly(dimethylsiloxane) (PDMS) layer containing separation and injection channels and an electrode plate. The copper microelectrode is fabricated by selective electroless deposition. The fabrication of the decoupler is performed by platinum electrochemical deposition on the metal film formed by electroless deposition. Factors influencing the performance, including detection potential, separation field strength, and buffer concentration, were studied. The electrodes exhibited good stability and durability in the analytical procedures. Under optimized detection conditions, glucose responded linearly from 10 microM to 1 mM. Finally, glucose in human plasma from three healthy individuals and two diabetics was successfully determined, giving a good prospect for a new clinical diagnostic instrument.

Full Text

Duke Authors

Cited Authors

  • Du, Y; Yan, J; Zhou, W; Yang, X; Wang, E

Published Date

  • November 2004

Published In

Volume / Issue

  • 25 / 21-22

Start / End Page

  • 3853 - 3859

PubMed ID

  • 15565683

Pubmed Central ID

  • 15565683

International Standard Serial Number (ISSN)

  • 0173-0835

Digital Object Identifier (DOI)

  • 10.1002/elps.200406072

Language

  • eng

Conference Location

  • Germany