Atomic force microscope investigation of large-circle DNA molecules.

Journal Article (Journal Article)

A circular bacterial artificial chromosome of 148.9kbp on human chromosome 3 has been extended and fixed on bare mica substrates using a developed fluid capillary flow method in evaporating liquid drops. Extended circular DNA molecules were imaged with an atomic force microscope (AFM) under ambient conditions. The measured total lengths of the whole DNA molecules were in agreement with sequencing analysis data with an error range of +/-3.6%. This work is important groundwork for probing single nucleotide polymorphisms in the human genome, mapping genomic DNA, manipulating biomolecular nanotechnology, and studying the interaction of DNA-protein complexes investigated by AFM.

Full Text

Duke Authors

Cited Authors

  • Wu, A; Yu, L; Li, Z; Yang, H; Wang, E

Published Date

  • February 15, 2004

Published In

Volume / Issue

  • 325 / 2

Start / End Page

  • 293 - 300

PubMed ID

  • 14751264

International Standard Serial Number (ISSN)

  • 0003-2697

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2003.11.005


  • eng

Conference Location

  • United States