Interferon increases the abundance of submembranous microfilaments in HeLa-S3 cells in suspension culture.

Journal Article (Journal Article)

Human beta (fibroblast) interferon inhibits the proliferation of human HeLa-S3 carcinoma cells in suspension culture. Accompanying this effect, the lateral mobility of cell surface receptors for concanavalin A is decreased and the rigidity of the plasma membrane lipid bilayer is increased. The present findings show a marked increase in the number of polymerized actin-containing microfilaments 3 days after treatment of HeLa-S3 cells with beta-interferon (640 units/ml). The cortical region of the treated enlarged cells contains a thick and dense meshwork of 40-70 A microfilaments. The actin nature of the filaments was verified by their ability to bind heavy meromyosin. These results support the concept that beta-interferon induces a coordinated response in the plasma membrane and the underlying microfilaments in both tumor and normal cells.

Full Text

Duke Authors

Cited Authors

  • Wang, E; Pfeffer, LM; Tamm, I

Published Date

  • October 1, 1981

Published In

Volume / Issue

  • 78 / 10

Start / End Page

  • 6281 - 6285

PubMed ID

  • 6171815

Pubmed Central ID

  • PMC349022

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.78.10.6281


  • eng

Conference Location

  • United States