Transcription profiling of estrogen target genes in young and old mouse uterus.

Published

Journal Article

The goal of this study was to identify age-related changes in the expression of estrogen target genes in mouse uterus. We developed a novel 'estrogen response element (ERE) Chip' microarray bearing 297 genes including both known estrogen target genes and genes identified by searching the mouse genome database to have EREs, AP-1 sites, and Sp1 sites, all targets of estrogen receptor (ER) regulation. 400-500 bp PCR products of these 297 genes were printed onto nylon membranes creating the 'ERE Chip' microarray. This microarray is unique because it is the first estrogen-responsive gene-specific microarray to identify changes in uterine gene expression in young versus old mice. Using this ERE microarray we identified 10 uterine genes whose expression was up-regulated in old mice, e.g. beta-actin, calcium binding protein 45a, Sp1, and COUP-TFII. In contrast, the expression of only 4 uterine genes, i.e., complement C3, lactoferrin, Muc-1, and 17-beta-hydroxysteroid dehydrogenase 8 (H2-Ke6) was down-regulated in old mice. These changes may reflect an increase in stromal and a decrease in glandular epithelial gene expression, and may be associated with age-related changes in these tissue compartments within the uterus, possibly leading to the decline in reproductive function in C57Bl/6 mice.

Full Text

Duke Authors

Cited Authors

  • Khalyfa, A; Klinge, CM; Hall, WC; Zhao, X; Miller, MM; Wang, E

Published Date

  • October 1, 2003

Published In

Volume / Issue

  • 38 / 10

Start / End Page

  • 1087 - 1099

PubMed ID

  • 14580862

Pubmed Central ID

  • 14580862

Electronic International Standard Serial Number (EISSN)

  • 1873-6815

International Standard Serial Number (ISSN)

  • 0531-5565

Digital Object Identifier (DOI)

  • 10.1016/s0531-5565(03)00210-9

Language

  • eng