Altered urinary bladder function in mice lacking the vanilloid receptor TRPV1.

Published

Journal Article

In the urinary bladder, the capsaicin-gated ion channel TRPV1 is expressed both within afferent nerve terminals and within the epithelial cells that line the bladder lumen. To determine the significance of this expression pattern, we analyzed bladder function in mice lacking TRPV1. Compared with wild-type littermates, trpv1(-/-) mice had a higher frequency of low-amplitude, non-voiding bladder contractions. This alteration was accompanied by reductions in both spinal cord signaling and reflex voiding during bladder filling (under anesthesia). In vitro, stretch-evoked ATP release and membrane capacitance changes were diminished in bladders excised from trpv1(-/-) mice, as was hypoosmolality-evoked ATP release from cultured trpv1(-/-) urothelial cells. These findings indicate that TRPV1 participates in normal bladder function and is essential for normal mechanically evoked purinergic signaling by the urothelium.

Full Text

Duke Authors

Cited Authors

  • Birder, LA; Nakamura, Y; Kiss, S; Nealen, ML; Barrick, S; Kanai, AJ; Wang, E; Ruiz, G; De Groat, WC; Apodaca, G; Watkins, S; Caterina, MJ

Published Date

  • September 2002

Published In

Volume / Issue

  • 5 / 9

Start / End Page

  • 856 - 860

PubMed ID

  • 12161756

Pubmed Central ID

  • 12161756

Electronic International Standard Serial Number (EISSN)

  • 1546-1726

International Standard Serial Number (ISSN)

  • 1097-6256

Digital Object Identifier (DOI)

  • 10.1038/nn902

Language

  • eng