Expression of an EF-1 alpha-like rat cDNA, S1, in E. coli and production of a rabbit polyclonal antiserum to the recombinant protein.

Journal Article (Journal Article)

A previously identified rat cDNA (S1) that shares 78% nucleotide homology and a predicted 92% amino acid homology with human EF-1 alpha was constructed into PGEX-2T and inducibly expressed in E. coli as a fusion protein. The purified recombinant S1 protein (rpS1) was used to raise rabbit antiserum which recognized rpS1 but not EF-1 alpha on immunoblots. A polyclonal antiserum to EF-1 alpha failed to react with the rpS1. Our results indicate that rpS1 protein can be used to produce antibodies which distinguish pS1 from EF-1 alpha despite their extensive amino acid sequence homology.

Full Text

Duke Authors

Cited Authors

  • Liu, CH; Liu, S; Wang, E

Published Date

  • September 30, 1993

Published In

Volume / Issue

  • 195 / 3

Start / End Page

  • 1371 - 1378

PubMed ID

  • 8216271

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1993.2195


  • eng

Conference Location

  • United States