Dynamic coating for resolving rhodamine B adsorption to poly(dimethylsiloxane)/glass hybrid chip with laser-induced fluorescence detection.

Published

Journal Article

In this paper a method was described about dynamic coating for resolving rhodamine B (RB) adsorption on a hybrid poly(dimethylsiloxane) (PDMS)/glass chip. The results showed that when the non-ionic surfactant Triton X-100 was higher than 0.5% (v/v) into the phosphate buffer, the adsorption of RB appeared. Besides, some separation conditions for RB were investigated, including concentration of Triton X-100, concentration and pH value of running buffer, separation voltage and detection site. Through comparing electroosmotic flow, plate numbers and other parameters, an acceptable separation condition was obtained. Under optimized conditions, the precisions of RB detection (R.S.D., n=10) were 2.62% for migration time, 4.78% for peak height respectively. Additionally, RB concentration linearity response was excellent with 0.9996 of correlation coefficient between 1 and 100muM, and a limit of detection (S/N=3) was 0.2muM. Finally, we separated rhodamine B isothiocyanate and lysine deriving from the fluorescent probe, and the result displayed that the dynamic coating method was applicable by CE separations using PDMS/glass chip.

Full Text

Duke Authors

Cited Authors

  • Kang, J; Yan, J; Liu, J; Qiu, H; Yin, X-B; Yang, X; Wang, E

Published Date

  • May 15, 2005

Published In

Volume / Issue

  • 66 / 4

Start / End Page

  • 1018 - 1024

PubMed ID

  • 18970086

Pubmed Central ID

  • 18970086

Electronic International Standard Serial Number (EISSN)

  • 1873-3573

Digital Object Identifier (DOI)

  • 10.1016/j.talanta.2005.01.002

Language

  • eng

Conference Location

  • Netherlands