Characterization of prolidase activity using capillary electrophoresis with tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence detection and application to evaluate collagen degradation in diabetes mellitus.
Journal Article (Journal Article)
A new method for prolidase (PLD, EC 3.4.13.9) activity assay was developed based on the determination of proline produced from enzymatic reaction through capillary electrophoresis (CE) with tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)3(2+)] electrochemiluminescence detection (ECL). A detection limit of 12.2 fmol (S/N = 3) for proline, corresponding to 1.22 x 10(-8) units of prolidase catalyzing for 1 min was achieved. PLD activity determined by CE-ECL method was in agreement with that obtained from the classical Chinard's one. CE-ECL showed its powerful resolving ability and selectivity as no sample pretreatment was needed and no interference existed. The clinical utility of this method was successfully demonstrated by its application to assay PLD activity in the serum of diabetic patients in order to evaluate collagen degradation in diabetes mellitus (DM). The results indicated that enhanced collagen degradation occurred in DM.
Full Text
Duke Authors
Cited Authors
- Yuan, J; Li, T; Yin, X-B; Guo, L; Jiang, X; Jin, W; Yang, X; Wang, E
Published Date
- May 1, 2006
Published In
Volume / Issue
- 78 / 9
Start / End Page
- 2934 - 2938
PubMed ID
- 16642978
International Standard Serial Number (ISSN)
- 0003-2700
Digital Object Identifier (DOI)
- 10.1021/ac051594x
Language
- eng
Conference Location
- United States