Peritoneal and subperitoneal stroma may facilitate regional spread of ovarian cancer.

Published

Journal Article

PURPOSE: Epithelial ovarian cancer (EOC) is characterized by early peritoneal involvement ultimately contributing to morbidity and mortality. To study the role of the peritoneum in fostering tumor invasion, we analyzed differences between the transcriptional repertoires of peritoneal tissue lacking detectable cancer in patients with EOC versus benign gynecologic disease. EXPERIMENTAL DESIGN: Specimens were collected at laparotomy from patients with benign disease (b) or malignant (m) ovarian pathology and comprised primary ovarian tumors, paired bilateral specimens from adjacent peritoneum and attached stroma (PE), subjacent stroma (ST), peritoneal washes, ascites, and peripheral blood mononuclear cells. Specimens were immediately frozen. RNA was amplified by in vitro transcription and cohybridized with reference RNA to a custom-made 17.5k cDNA microarray. RESULTS: Principal component analysis and unsupervised clustering did not segregate specimens from patients with benign or malignant pathology. Class comparison identified differences between benign and malignant PE and ST specimens deemed significant by permutation test (P = 0.027 and 0.012, respectively). A two-tailed Student's t test identified 402 (bPE versus mPE) and 663 (mST versus bST) genes differentially expressed at a significance level of P2 < or = 0.005 when all available paired samples from each patient were analyzed. The same comparison using one sample per patient reduced the pool of differentially expressed genes but retained permutation test significance for bST versus mST (P = 0.031) and borderline significance for bPE versus mPE (P = 0.056) differences. CONCLUSIONS: The presence of EOC may foster peritoneal implantation and growth of cancer cells by inducing factors that may represent molecular targets for disease control.

Full Text

Duke Authors

Cited Authors

  • Wang, E; Ngalame, Y; Panelli, MC; Nguyen-Jackson, H; Deavers, M; Mueller, P; Hu, W; Savary, CA; Kobayashi, R; Freedman, RS; Marincola, FM

Published Date

  • January 1, 2005

Published In

Volume / Issue

  • 11 / 1

Start / End Page

  • 113 - 122

PubMed ID

  • 15671535

Pubmed Central ID

  • 15671535

International Standard Serial Number (ISSN)

  • 1078-0432

Language

  • eng

Conference Location

  • United States