Electrochemiluminescence quenching as an indirect method for detection of dopamine and epinephrine with capillary electrophoresis.

Journal Article (Journal Article)

An electrochemiluminescence (ECL) inhibition method was developed as an indirect detection method for the determination of dopamine and epinephrine separated by capillary electrophoresis (CE). When the concentration of Ru(bpy)(3) (2+) was 50 muM diluted by 50 mM phosphate (pH 8.5) in the cell and 0.5 M tripropylamine (TPA) was added to the running buffer (10 mM phosphate, pH 9.0), an inhibition of ECL of the Ru(bpy)(3) (2+)/TPA system by the analytes was observed. Under the optimized conditions, the relative standard deviations of migration time and negative peak area were less than 1% and 3%, respectively, for 1 microM dopamine or 1 microM epinephrine (n = 10). Linear ranges of 0.1-10 microM for both analytes and the detection limits (signal-to-noise ratio S/N = 3) of 10 nM for dopamine and 30 nM for epinephrine were obtained.

Full Text

Duke Authors

Cited Authors

  • Kang, J; Yin, X-B; Yang, X; Wang, E

Published Date

  • May 2005

Published In

Volume / Issue

  • 26 / 9

Start / End Page

  • 1732 - 1736

PubMed ID

  • 15812842

International Standard Serial Number (ISSN)

  • 0173-0835

Digital Object Identifier (DOI)

  • 10.1002/elps.200410247


  • eng

Conference Location

  • Germany