A novel, rapid assay for detection and differentiation of serotype-specific antibodies to Venezuelan equine encephalitis complex alphaviruses.

Published

Journal Article

An epitope-blocking enzyme-linked immunosorbent assay was developed for the rapid differentiation of serologic responses to enzootic variety IE and ID versus epizootic variety IAB and IC strains of Venezuelan equine encephalitis (VEE) virus. Two monoclonal antibodies that differentially recognize epizootic versus enzootic VEE virus epitopes were used to measure the serotype-specific blocking abilities of antibodies in sera of naturally infected humans, equines, and bovines, as well as in experimentally infected equines. The assay is simple, species-independent, rapid, and sensitive, and will improve surveillance for VEE emergence. It could also be used to determine the epidemic potential of a VEE virus following an intentional introduction for bioterrorism.

Full Text

Duke Authors

Cited Authors

  • Wang, E; Paessler, S; Aguilar, PV; Smith, DR; Coffey, LL; Kang, W; Pfeffer, M; Olson, J; Blair, PJ; Guevara, C; Estrada-Franco, J; Weaver, SC

Published Date

  • June 2005

Published In

Volume / Issue

  • 72 / 6

Start / End Page

  • 805 - 810

PubMed ID

  • 15964967

Pubmed Central ID

  • 15964967

International Standard Serial Number (ISSN)

  • 0002-9637

Language

  • eng

Conference Location

  • United States