Enhanced surface plasmon resonance immunosensing using a streptavidin-biotinylated protein complex.

Published

Journal Article

In this paper, we present a novel strategy for improving the sensitivity of surface plasmon resonance immunosensing using a streptavidin-biotinylated protein complex. This amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. The complex can be formed in a cross-linking network of molecules so that the amplification of the response signal will be realized due to the big molecular size of the complex. The results show that the amplification strategy causes a dramatic improvement of the detection sensitivity. hIgG protein could be detected in the range of 0.005-10 micrograms ml-1.

Full Text

Duke Authors

Cited Authors

  • Pei, R; Yang, X; Wang, E

Published Date

  • January 2001

Published In

Volume / Issue

  • 126 / 1

Start / End Page

  • 4 - 6

PubMed ID

  • 11205509

Pubmed Central ID

  • 11205509

International Standard Serial Number (ISSN)

  • 0003-2654

Digital Object Identifier (DOI)

  • 10.1039/b007432m

Language

  • eng

Conference Location

  • England