Construction and control of plasmid DNA network.

Published

Journal Article

The influences of different cations on plasmid DNA network structures on a mica substrate were investigated by atomic force microscopy (AFM). Interactions between the DNA strands and mica substrate, and between the DNA strands themselves were more strongly influenced by the complex cations (Fe(phen)3(2+), Ni(phen)3(2+), and Co(phen)3(3+)) than by the simple cations (Mg2+, Mn2+, Ni2+, Ca2+, Co3+). The mesh height of the plasmid DNA network was higher when the complex cations were added to DNA samples. The mesh size decreased with increasing DNA concentration and increased with decreasing DNA concentration in the same cation solution sample. Hence, plasmid DNA network height can be controlled by selecting different cations, and the mesh size can be controlled by adjusting plasmid DNA concentration.

Full Text

Duke Authors

Cited Authors

  • Wu, A; Li, Z; Zhou, H; Zheng, J; Wang, E

Published Date

  • May 2002

Published In

Volume / Issue

  • 127 / 5

Start / End Page

  • 585 - 587

PubMed ID

  • 12081032

Pubmed Central ID

  • 12081032

International Standard Serial Number (ISSN)

  • 0003-2654

Language

  • eng

Conference Location

  • England