Electrocatalytic oxidation and flow detection of cysteine at an aquocobalamin adsorbed glassy carbon electrode.


Journal Article

A chemically modified electrode (CME) constructed by adsorption of aquocobalamin (VB12a) onto a glassy carbon electrode surface was demonstrated to catalyze the electro-oxidation of cysteine, a sulfhydryl-containing compound. The sulfhydryl oxidation occurred at 0.54-0.88 V vs. Ag AgCl depending on pH value (3.0-10.0). The electrocatalytic behavior of cysteine is elucidated with respect to solution pH, operating potential and other variables as well as the CME preparation conditions. When used as the sensing electrode in flow injection amperometric detection, the CME permitted detection of the compound at 0.8 V. The detection limit was 1.7 pmol. The linear response range went up to 1.16 nmol. The stability of the CME was shown by RSD (4.2%) over 10 repeated injections.

Full Text

Duke Authors

Cited Authors

  • Li, H; Li, T; Wang, E

Published Date

  • July 1995

Published In

Volume / Issue

  • 42 / 7

Start / End Page

  • 885 - 890

PubMed ID

  • 18966306

Pubmed Central ID

  • 18966306

International Standard Serial Number (ISSN)

  • 0039-9140

Digital Object Identifier (DOI)

  • 10.1016/0039-9140(95)01502-3


  • eng

Conference Location

  • Netherlands