Duplication of single stranded DNA catalyzed by calf thymus DNA polymerase alpha.
Journal Article (Journal Article)
Purified calf thymus DNA polymerase alpha is inactive with native DNA as template and shows little activity with denatured DNA. DNA synthesis with denatured DNA as template is greatly stimulated by the addition of a nuclease which initially copurifies with DNA polymerase but is separated from the polymerase on DEAE-cellulose chromatography. A limit digest of nuclease treated native DNA which is then denatured is replicated 80-95%; extensive replication is also obtained with native DNA partially degraded by pancreatic DNase and then denatured. The product of the reaction with calf thymus nuclease-treated DNA as template is double-stranded DNA with a hairpin (looped back) structure.
Full Text
Duke Authors
Cited Authors
- Wang, E; Henner, D; Furth, JJ
Published Date
- January 1976
Published In
Volume / Issue
- 3 / 1
Start / End Page
- 129 - 147
PubMed ID
- 175352
Pubmed Central ID
- PMC342883
International Standard Serial Number (ISSN)
- 0305-1048
Digital Object Identifier (DOI)
- 10.1093/nar/3.1.129
Language
- eng
Conference Location
- England